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[2017 08 11] 특별세미나 _Prof. Ki Soo Park (Konkuk University)

Advanced biosensors for rapid diagnosis of infection

관리자 | 2017.08.07 11:40 | 조회 277


 

Advanced biosensors for rapid diagnosis of infection

 

Date: 2017. 08. 11(Fri) 16:00 ~ 17:30

Place: Science Building, Room.202 Multimedia Room

Speaker: Prof. Ki Soo Park (Konkuk University)

Host of a Seminar: Prof. Seung Soo Oh

Abstract:

 

Bacterial infections and the emergence of drug-resistant pathogens are major healthcare issues with millions of reported cases every year. Advanced diagnostics would allow clinicians to more quickly determine the most effective treatment, reduce the empirical use of broad-spectrum antimicrobials, and facilitate enrollment in new antibiotic treatments. In this talk, I will describe new approaches for rapid diagnosis of infection. In the first part of this talk, I will present a new integrated system, PAD (polarization anisotropy diagnostics), for rapid bacterial detection. The PAD assay detects PCR-amplified pathogenic DNAs that are captured in a sequence specific manner by carefully designed detection keys. This hybridization event causes the detection keys to lock DNA polymerase, which alters the fluorescence anisotropy of a separate reporter probe. Due to this unique feature of the detection keys, the signal readout can be performed in a simple homogenous format. By targeting the conserved and hypervariable regions of 16S rRNA, the universal and differential detection of pathogenic bacteria was achieved with sensitivity down to a single bacterium. In addition, drug-resistant pathogens were distinguished by detecting the drug-resistance gene markers. The technology is inherently robust against environmental noise, and economically scalable for parallel measurements. The assay is fast (2 hours) and performed on-site in a single tube format. When applied to clinical samples from interventional procedures, PAD determined the overall bacterial burden, differentiated bacterial species, and identified drug resistance and virulence status. The PAD system holds promise as a powerful tool for near-patient, rapid bacterial testing. In the second part of this talk, I will present a new integrated magnetic approach, MCD (magnetic capture and detection) to enable early detection of infectious diseases. Pathogenic infection activates B cells and induces them to produce antigen-specific antibodies. I reasoned that these secreted antibodies could serve as an early biomarker for infection. I thus developed a nanoparticle-based assay wherein antibody-secreting cells (ASCs) are enriched through magnetic capture, and secreted antibodies are magnetically detected by a miniaturized nuclear magnetic resonance (µNMR) system. This approach is based entirely on magnetics which supports high contrast against biological background, and simplifies assay procedures. TheMCD systemis advancedby i) synthesizing magnetic nanoparticles (MNPs)with high magnetic momentsforbothcell capture and antibody detection, ii) developing a new magnetic structure for high-yield cell capture, and iii) optimizing the µNMR assay for antibody detection.As a proof-of-concept, MCD is applied to detect antibodies produced by Salmonellaspecific ASCs.The MCD achieved high sensitivity in detecting antibodies secreted from as few as 5 ASCs (50cells/mL). Importantly, the assay could be performed with whole blood with minimal sample processing.The MCD platform has the potential to be a powerful first-response tool for early infection diagnosis and this approach can potentially be applied to a number of pathogens(e.g. Zika, influenza and ebola).

 

 

Dept. of MSE / BK21+

 




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